Isolation of Chioroquine-Resistant Chinese Hamster V79 Cell Variants that Are Also Resistant to Aminonium Chloride
Identifieur interne : 003633 ( Main/Exploration ); précédent : 003632; suivant : 003634Isolation of Chioroquine-Resistant Chinese Hamster V79 Cell Variants that Are Also Resistant to Aminonium Chloride
Auteurs : Mayumi Ono ; Miho Ando ; Tatsuo Shimada ; Koji Furuno ; Keitaro Kato ; Michihiko KuwanoSource :
- The Journal of Biochemistry [ 0021-924X ] ; 1983.
Abstract
Chloroquine-resistant (CQr) clones (CQ-21 and CQ-22) have been isolated from mutagenized hamster lung V79 cells by exposing the cells to a high dose of chloroquine. CQ-21 and CQ-22 showed about 3-fold higher resistance to chloroquine than the parental V79 cells, and they showed specific cross-resistance to another amine, NH4Cl, which is also concentrated in lysosomes. CQr clone showed no cross-resistance to other unrelated agents. Chloroquine-induced inhibition of [125I]ricin internalization was observed in both cell lines at neutral pH, but the inhibition of uptake was less in the variant. Also, the degradation of endogenous protein was slowed in the mutant; further, treatment of cells with 30 μg/ml of chloroquine inhibited the degradation of endogenous proteins in the parental V79, but not in CQ-22 cells. Similar levels of acid phosphatase, β-glucuronidase and cathepsin D were observed in V79 and CQ-22 cells, but the level of cathepsin B was lower in the mutant. Electron microscopy showed an increased number of electron-dense bodies, possibly autophagosomes/lysosomes, in the mutant cells grown for 4 days with 5 μg/ml of chloroquine. Similar aberrant structures were observed in the parental V79 cells treated for only 3 h with 5 μg/ml of chloroquine.
Url:
DOI: 10.1093/oxfordjournals.jbchem.a134497
Affiliations:
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<record><TEI wicri:istexFullTextTei="biblStruct"><teiHeader><fileDesc><titleStmt><title>Isolation of Chioroquine-Resistant Chinese Hamster V79 Cell Variants that Are Also Resistant to Aminonium Chloride</title><author><name sortKey="Ono, Mayumi" sort="Ono, Mayumi" uniqKey="Ono M" first="Mayumi" last="Ono">Mayumi Ono</name></author><author><name sortKey="Ando, Miho" sort="Ando, Miho" uniqKey="Ando M" first="Miho" last="Ando">Miho Ando</name></author><author><name sortKey="Shimada, Tatsuo" sort="Shimada, Tatsuo" uniqKey="Shimada T" first="Tatsuo" last="Shimada">Tatsuo Shimada</name></author><author><name sortKey="Furuno, Koji" sort="Furuno, Koji" uniqKey="Furuno K" first="Koji" last="Furuno">Koji Furuno</name></author><author><name sortKey="Kato, Keitaro" sort="Kato, Keitaro" uniqKey="Kato K" first="Keitaro" last="Kato">Keitaro Kato</name></author><author><name sortKey="Kuwano, Michihiko" sort="Kuwano, Michihiko" uniqKey="Kuwano M" first="Michihiko" last="Kuwano">Michihiko Kuwano</name></author></titleStmt><publicationStmt><idno type="wicri:source">ISTEX</idno><idno type="RBID">ISTEX:B84A2608293581F141EEAC75E21EBA07F7FA796F</idno><date when="1983" year="1983">1983</date><idno type="doi">10.1093/oxfordjournals.jbchem.a134497</idno><idno type="url">https://api.istex.fr/ark:/67375/HXZ-3WS54V48-T/fulltext.pdf</idno><idno type="wicri:Area/Istex/Corpus">000930</idno><idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Corpus" wicri:corpus="ISTEX">000930</idno><idno type="wicri:Area/Istex/Curation">000930</idno><idno type="wicri:Area/Istex/Checkpoint">002393</idno><idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Checkpoint">002393</idno><idno type="wicri:doubleKey">0021-924X:1983:Ono M:isolation:of:chioroquine</idno><idno type="wicri:Area/Main/Merge">003710</idno><idno type="wicri:Area/Main/Curation">003633</idno><idno type="wicri:Area/Main/Exploration">003633</idno></publicationStmt><sourceDesc><biblStruct><analytic><title level="a" type="main" xml:lang="en">Isolation of Chioroquine-Resistant Chinese Hamster V79 Cell Variants that Are Also Resistant to Aminonium Chloride<ref type="fn" target="#fn1"><hi rend="superscript">1</hi></ref></title><author><name sortKey="Ono, Mayumi" sort="Ono, Mayumi" uniqKey="Ono M" first="Mayumi" last="Ono">Mayumi Ono</name><affiliation></affiliation><affiliation></affiliation><affiliation><wicri:noCountry code="no comma">2To whom correspondence should be sent.</wicri:noCountry></affiliation></author><author><name sortKey="Ando, Miho" sort="Ando, Miho" uniqKey="Ando M" first="Miho" last="Ando">Miho Ando</name><affiliation><wicri:noCountry code="subField">879-56</wicri:noCountry></affiliation></author><author><name sortKey="Shimada, Tatsuo" sort="Shimada, Tatsuo" uniqKey="Shimada T" first="Tatsuo" last="Shimada">Tatsuo Shimada</name><affiliation><wicri:noCountry code="subField">879-56</wicri:noCountry></affiliation></author><author><name sortKey="Furuno, Koji" sort="Furuno, Koji" uniqKey="Furuno K" first="Koji" last="Furuno">Koji Furuno</name><affiliation><wicri:noCountry code="subField">812</wicri:noCountry></affiliation></author><author><name sortKey="Kato, Keitaro" sort="Kato, Keitaro" uniqKey="Kato K" first="Keitaro" last="Kato">Keitaro Kato</name><affiliation><wicri:noCountry code="subField">812</wicri:noCountry></affiliation></author><author><name sortKey="Kuwano, Michihiko" sort="Kuwano, Michihiko" uniqKey="Kuwano M" first="Michihiko" last="Kuwano">Michihiko Kuwano</name><affiliation><wicri:noCountry code="subField">879-56</wicri:noCountry></affiliation></author></analytic><monogr></monogr><series><title level="j" type="main">The Journal of Biochemistry</title><idno type="eISSN">-</idno><idno type="ISSN">0021-924X</idno><imprint><publisher>Oxford University Press</publisher><date when="1983-11">1983</date><biblScope unit="vol">94</biblScope><biblScope unit="issue">5</biblScope><biblScope unit="page" from="1493">1493</biblScope><biblScope unit="page" to="1503">1503</biblScope></imprint><idno type="ISSN">0021-924X</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0021-924X</idno></seriesStmt></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract">Chloroquine-resistant (CQr) clones (CQ-21 and CQ-22) have been isolated from mutagenized hamster lung V79 cells by exposing the cells to a high dose of chloroquine. CQ-21 and CQ-22 showed about 3-fold higher resistance to chloroquine than the parental V79 cells, and they showed specific cross-resistance to another amine, NH4Cl, which is also concentrated in lysosomes. CQr clone showed no cross-resistance to other unrelated agents. Chloroquine-induced inhibition of [125I]ricin internalization was observed in both cell lines at neutral pH, but the inhibition of uptake was less in the variant. Also, the degradation of endogenous protein was slowed in the mutant; further, treatment of cells with 30 μg/ml of chloroquine inhibited the degradation of endogenous proteins in the parental V79, but not in CQ-22 cells. Similar levels of acid phosphatase, β-glucuronidase and cathepsin D were observed in V79 and CQ-22 cells, but the level of cathepsin B was lower in the mutant. Electron microscopy showed an increased number of electron-dense bodies, possibly autophagosomes/lysosomes, in the mutant cells grown for 4 days with 5 μg/ml of chloroquine. Similar aberrant structures were observed in the parental V79 cells treated for only 3 h with 5 μg/ml of chloroquine.</div></front></TEI><affiliations><list></list><tree><noCountry><name sortKey="Ando, Miho" sort="Ando, Miho" uniqKey="Ando M" first="Miho" last="Ando">Miho Ando</name><name sortKey="Furuno, Koji" sort="Furuno, Koji" uniqKey="Furuno K" first="Koji" last="Furuno">Koji Furuno</name><name sortKey="Kato, Keitaro" sort="Kato, Keitaro" uniqKey="Kato K" first="Keitaro" last="Kato">Keitaro Kato</name><name sortKey="Kuwano, Michihiko" sort="Kuwano, Michihiko" uniqKey="Kuwano M" first="Michihiko" last="Kuwano">Michihiko Kuwano</name><name sortKey="Ono, Mayumi" sort="Ono, Mayumi" uniqKey="Ono M" first="Mayumi" last="Ono">Mayumi Ono</name><name sortKey="Shimada, Tatsuo" sort="Shimada, Tatsuo" uniqKey="Shimada T" first="Tatsuo" last="Shimada">Tatsuo Shimada</name></noCountry></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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